My research is in the field of biological sciences and agriculture, with research interests in glyco-engineering and enzyme engineering, as well as plant glycobiology and plant immunity. My research focuses on the utilization of polysaccharide biomass resources in nature, which includes two main aspects: mining and creation of polysaccharide degrading enzymes and efficient degradation of polysaccharides; and the functions and mechanisms of oligosaccharides (polysaccharide degradation products), especially the regulatory mechanisms of oligosaccharides on plant immune system.
I have discovered and created a series of glycoside hydrolases, polysaccharide lyases, LPMO and other efficient polysaccharide degradation-related enzymes, and conducted in-depth studies on the catalytic mechanism of the enzymes; based on the above-mentioned enzymes with independent intellectual property rights, I have realized the preparation of a series of functional oligosaccharides; I have conducted systematic studies on the functions and mechanisms of oligosaccharides in regulating the plant immune system in various crops. I have published more than 120 research papers in Appl Catal B, Plant J, ACS Catal, J Phys Chem Lett, Carbohyd Polym, Bioresource Technol, Food Chem, AEM, JAFC, etc. I have published 3 books and several book chapters. I have been granted more than 40 patents and have translated more than 10 patents into corporate industrial applications. I have collaborated with companies to achieve the industrial application of polysaccharide degradation technology and developed more than 20 kinds of biopesticides and biofertilizers with oligosaccharides as active ingredients, which have been widely used in China and abroad.
1. 2020 Liaoning Province “One Hundred Talent Project” - Hundred People Level
2. 2019 “Zhang Dayu” Excellent Prof. of DICP
3. 2019 Outstanding Member of the Youth Association of the Chinese Academy of Sciences
4. 2018 Liaoning Revitalization Talents
5. 2016 Liaoning Province “One Hundred Talent Project” - Thousand People Level
6. 2015 Distinguished Young Scientists of Dalian city
7. 2015 Zhang Shuzheng Glycoscience Award for Outstanding Youth
8. 2014 The second-class award of Liaoning provincial prize for progress in science and technology
9. 2014 Youth Star science and technology researcher of Dalian city
10. 2013 The first-class award of Marine Science and Technology Award of China
11. 2013 YSP Award of Asian Community of Glycoscience and Glycotechnology
12.2010 Denmark DANIDA Fellowship
Book and Book chapter
1. Heng Yin, Yuguang Du, Research Progress in Oligosaccharins, ISBN 978‐1‐4939‐3518‐5, Publisher:
Springer, 2016
Journal papers (In recent 5 years)
1. Xiaochen Jia, Hongyan Zhang, Hongqiang Qin, Kuikui Li, Xiaoyan Liu, Wenxia Wang, Mingliang Ye, Heng Yin. Protein O-GlcNAcylation impairment caused by N-acetylglucosamine phosphate mutase deficiency leads to growth variations in Arabidopsis thaliana. The Plant Journal, 2023, 114(3):613-635.
2. Yijie Dong, Tang Li, Shiqing Zhang, Joaquin Sanchis, Heng Yin, Jie Ren, Xiang Sheng, Guangyue Li, Manfred T. Reetz. Biocatalytic baeyer-villiger reactions: Uncovering the source of regioselectivity at each evolutionary stage of a mutant with scrutiny of fleeting chiral intermediates. ACS Catalysis, 2022, 12(6): 3669-3680.
3. Lingfeng Long, Yun Hu, Fubao Sun, Wa Gao, Zhikui Hao, Heng Yin. Advances in lytic polysaccharide monooxygenases with the cellulose-degrading auxiliary activity family 9 to facilitate cellulose degradation for biorefinery. International Journal of Biological Macromolecules, 2022, 219: 68-83.
4. En Yu, Daiwen Chen, Bing Yu, Zhiqing Huang, Xiangbing Mao, Ping Zheng, Yuheng Luo, Heng Yin, Jie Yu, Junqiu Luo, Hui Yan, Jun He. Alteration of porcine intestinal microbiota in response to dietary manno-oligosaccharide supplementation. Frontiers in Microbiology, 2022, 12: 811272(1-11).
5. Wa Gao, Hefeng Zhang, Tang Li, Jiu Ju, Haichuan Zhou, Xu Zong, Heng Yin. Controlled depolymerization of cellulose by photoelectrochemical bioreactor using a lytic polysaccharide monooxygenase. Biochemical Engineering Journal, 2022, 187: 108597(1-6).
6. Can Zhao, Chunyan Wu, Kuikui Li, John F. Kennedy, Michael Wisniewski, Lihong Gao，Chenggui Han, Jia Liu, Heng Yin, Xuehong Wu. Effect of oligogalacturonides on seed germination and disease resistance of sugar beet seedling and root. Journal of Fungi, 2022, 8(716): 1-13.
7. Ruixin Li, Maolong Li, Jinxia He, Hongguo Xie, Wenxia Wang, Meng Zhang, Gustavo Cabrera-Barjas, Pierfrancesco Morganti, Heng Yin. Preparation of pectin nanospheres and its effect on wheat (Triticum aestivum L.) seed germination and growth. Journal of Plant Growth Regulation, 2022. 41: 3197-3207.
8. Xiao Yin, HongguoXie, Ruixin Li, Shenggang Yan, Heng Yin. Regulating association strength between quaternary ammonium chitosan and sodium alginate via hydration. Carbohydrate Polymers. 2021, 255: 117390(1-8).
9. Jin Wan, Jiao Zhang, Qingsong Xu, Heng Yin, Daiwen Chen, Bing Yu, Jun He. Alginate oligosaccharide protects against enterotoxigenic Escherichia coli-induced porcine intestinal barrier injury. Carbohydrate Polymers. 2021, 270: 118316(1-12).
10. Tang Li, Ningning Zhang, Shenggang Yan, Shan Jiang, Heng Yin. A novel tyrosinase from armillariaostoyae with comparable monophenolase and diphenolase activities suffers substrate inhibition. Applied and Environmental Microbiology. 2021, 87(12): 1-14.
11. Guojun Yang, Shuguang Li, Haidong Tan, Kuikui Li, Wei Chen, Heng Yin. A highly efficient biocatalytic conversion of renewable pectic polysaccharide biomass into bioactive oligogalacturonides. ACS Food Science & Technology. 2021, 1(3): 338-346.
12. Yu Wang, Xue Sun, XiaochenJia, Liping Zhu, Heng Yin. Comparative transcriptomic of Stevia rebaudiana provides insight into rebaudioside D and rebaudioside M biosynthesis. Plant Physiology and Biochemistry. 2021, 167: 541-549.
13. Santosh Kumar Bose, Yanqiu He, Prianka Howlader, Wenxia Wang, Heng Yin. The N-glycan processing enzymes beta-D-N-acetylhexosaminidase are involved in ripening-associated softening in strawberry fruit. Journal of Food Science and Technology. 2021, 58: 621-631.
14. Qian Li, Chaofeng Jiang, Haidong Tan, Xiaoming Zhao, Kuikui Li, Heng Yin. Characterization of recombinant E. coli expressing a novel fucosidase from Bacillus cereus 2-8 belonging to GH95 family. Protein Expression and Purification. 2021, 105897: 1-7.
15. En Yu, Daiwen Chen, Bing Yu, Yuheng Luo, Ping Zheng, Heng Yin, Xiangbing Mao, Zhiqing Huang, Jie Yu, Junqiu Luo, HuiYan, Jun He. Amelioration of enterotoxigenic Escherichia coli-induced disruption of intestinal epithelium by manno-oligosaccharide in weaned pigs. Journal of Functional Foods. 2021, 82: 104492(1-10).
16. Gustavo Cabrera-Barjas, Cristian González, Aleksandra Nesic, Kelly P. Marrugo, Oscar Gómez, Cédric Delattre, Oscar Valdes, Heng Yin, Gaston Bravo, Juan Cea. Utilization of marine waste to obtain β-chitin nanofibers and films from giant humboldt squid Dosidicus gigas. Marine Drugs. 2021, 19(4): 184.
17. En Yu, Daiwen Chen, Bing Yu, Zhiqing Huang, Xiangbing Mao, Ping Zheng, Yuheng Luo, Heng Yin, Jie Yu, Junqiu Luo, Hui Yan, Jun He. Manno-oligosaccharide attenuates inflammation and intestinal epithelium injury in weaned pigs upon enterotoxigenic Escherichia coli K88 challenge. British Journal of Nutrition. 2021, 126(7): 993-1002.
18. Hefeng Zhang, Yong Zhao, Huiyan Zhang, Haichuan Zhou, Hong Wang, Xu Zong, Heng Yin, Can Li. Establishing inorganic-biological hybrid photoelectrochemical platform towards sustainable conversion of α-chitin. Applied Catalysis B: Environmental. 2020, 265: 118558.
19. Guojun Yang, Haidong Tan, Shuguang Li, Meng Zhang, Jia Che, Kuikui Li, Wei Chen, Heng Yin. Application of engineered yeast strain fermentation for oligogalacturonides production from pectin-rich waste biomass. Bioresource Technology. 2020, 300: 122645.
20. Xiaochen Jia, Haihong Zeng, Santosh Kumar Bose, Wenxia Wang, Heng Yin. Chitosan oligosaccharide induces resistance to Pst DC3000 in Arabidopsis via a non-canonical N-glycosylation regulation pattern. Carbohydrate Polymers. 2020, 250: 116939.
21. Haichuan Zhou, Yuebin Zhang, Tang Li, Haidong Tan, Guohui Li, Heng Yin. Distinct Interaction of Lytic Polysaccharide Monooxygenase with Cellulose Revealed by Computational and Biochemical Studies. Journal of Physical Chemistry Letters. 2020,11: 3987-3992.
22. Jia Liu, John F. Kennedy, Xiaofang Zhang, Heng Yin, Wei Chen, Zhuo Chen, Xian Wu, Xuehong Wu. Preparation of alginate oligosaccharide and its effects on decay control and quality maintenance of harvested kiwifruit. Carbohydrate Polymers. 2020,242: 116462.
23. Santosh Kumar Bose, Prianka Howlader, Wenxia Wang, Heng Yin. Oligosaccharide is a promising natural preservative for improving postharvest preservation of fruit: A review. Food Chemistry. 2020, 341: 128178.
24. Guojun Yang, Wei Chen, Haidong Tan, Kuikui Li, Junyan Li, Heng Yin. Biochemical characterization and evolutionary analysis of a novel pectate lyase from Aspergillus parasiticus. International Journal of Biological Macromolecules. 2020, 152: 180-188.
25. Junjie Liu, Chunguang Zhang, Xiaochen Jia, Wenxia Wang, Heng Yin. Comparative analysis of RNA-binding proteomes under Arabidopsis thaliana-Pst DC3000-PAMP interaction by orthogonal organic phase separation. International Journal of Biological Macromolecules. 2020, 160: 47-54.
26. Prianka Howlader, Santosh Kumar Bose, Xiaochen Jia, Chunlai Zhang, Wenxia Wang, Heng Yin. Oligogalacturonides induce resistance in Arabidopsis thaliana by triggering salicylic acid and jasmonic acid pathways against Pst DC3000. International Journal of Biological Macromolecules. 2020, 164: 4054-4064.
27. Ming Sun, Chu Sun, Tang Li, Kuikui Li, Shenggang Yan, Heng Yin. Characterization of a novel bifunctional mannuronan C-5 epimerase and alginate lyase from Pseudomonas mendocina. sp. DICP-70. International Journal of Biological Macromolecules. 2020, 150: 662–670.
28. Junyan Ma, Tang Li, Haidong Tan, Wujun Liu, Heng Yin. The important roles played in substrate binding of aromatic amino acids in exo-inulinase from Kluyveromyces cicerisporus CBS 4857. Frontiers in Molecular Biosciences. 2020, 7: 569797.
29. Fu Hu, Benwei Zhu, Qian Li, Heng Yin, Yun Sun, Zhong Yao, Dengming Ming. Elucidation of a unique pattern and the role of carbohydrate binding module of an alginate lyase. Marine Drugs. 2020, 18, 32.
30. Xiaochen Jia, Hongqiang Qin, Santosh Kumar Bose, Tongmei Liu, Jinxia He, Shangqiang Xie, Mingliang Ye, Heng Yin. Proteomics analysis reveals the defense priming effect of chitosan oligosaccharides in Arabidopsis-Pst DC3000 interaction. Plant Physiology and Biochemistry. 2020, 149: 301-312.
31. Kuikui Li, Huan Qi, Qishun Liu, Tang Li, Wei Chen, Shuguang Li, Hai-long Piao, Heng Yin. Preparation and antitumor activity of selenium-modified glucomannan oligosaccharides. Journal of Functional Foods.2020, 65: 103731.
32. Shuli Wu, Qishun Liu, Haidong Tan, Fuyun Zhang, Heng Yin. A novel 2,5-furandicarboxylic acid biosynthesis route from biomass-derived 5-hydroxymethylfurfural based on the consecutive enzyme reactions. Applied Biochemistry and Biotechnology. 2020, 191: 1470-1482.
33. Xiaochen Jia, Mijanur Rahman Rajib, Heng Yin. Recognition pattern, functional mechanism and application of chitin and chitosan oligosaccharides in sustainable agriculture. Current Pharmaceutical Design. 2020, 26: 1-14.
34. Chunguang Zhang, Wenxia Wang, Xiaoming Zhao, Hongying Wang, Heng Yin. Preparation of alginate oligosaccharides and their biological activities in plants: A review. Carbohydrate Research. 2020, 494: 108056.
35. Haidong Tan, Heng Yin. Optimization and characterization of oligosaccharides production from citrus peel waste resource using Aspergillus niger 1805. Journal of Microbiological Methods. 2020, 169: 105809.
36. Haidong Tan, Guojun Yang, Wei Chen, Qishun Liu, Kuikui Li, Heng Yin. Identification and characterization of thermostable endo-polygalacturonase II B from Aspergillus luchuensis. Jouranal of Food Biochemistry. 2020, 13133.
37. Prianka Howlader, Santosh Kumar Bose, Chunguang Zhang, Xiaochen Jia, Wenxia Wang, Heng Yin. Pectin oligosaccharide (POS) induce resistance in Arabidopsis thaliana through salicylic acid-mediated signaling pathway against Pseudomonas syringae pv.tomato DC3000. Physiological and Molecular Plant Pathology. 2020, 110: 101483.
38. Haidong Tan, Heng Yin. Optimization and characterization of oligosaccharides production from citrus peel waste resource using Aspergillus niger 1805. Journal Of Microbiological Methods. 2020, 169(105): 809.
39. Haidong Tan, Guojun Yang, Wei Chen, Qishun Liu, Kuikui Li, Heng Yin. Identification and characterization of thermostable endo‐polygalacturonase II B from Aspergillus luchuensis. Journal of Food Biochemistry. 2020, 44(3): e13133.
40. Chunguang Zhang, Prianka Howlader, Tongmei Liu, Xue Sun, Xiaochen Jia, Xiaoming Zhao, Peili Shen, Yimin Qin, Wenxia Wang, Heng Yin. Alginate Oligosaccharide (AOS) induced resistance to Pst DC3000 via salicylic acid-mediated signaling pathway in Arabidopsis thaliana. Carbohydrate Polymers. 2019, 225(115): 221.
41. Qishun Liu, Changhong Yao, Yongxin Sun, Wei Chen, Haidong Tan, Xupeng Cao, Song Xue, Heng Yin. Production and structural characterization of a new type of polysaccharide from nitrogen-limited Arthrospira platensis cultivated in outdoor industrial-scale open raceway ponds. Biotechnology For Biofuels. 2019, 12: 131.
42. Santosh kumar Bose, Prianka Howlader, Xiaochen Jia, Wenxia Wang, Heng Yin. Alginate oligosaccharide postharvest treatment preserve fruit quality and increase storage life via Abscisic acid signaling in strawberry. Food Chemistry. 2019, 283: 665-674.
43. Haichuan Zhou, Tang Li, Zuochen Yu, Jiu Ju, Huiyan Zhang, Haidong Tan, Kuikui Li, Heng Yin. A lytic polysaccharide monooxygenase from Myceliophthora thermophila and its synergism with cellobiohydrolases in cellulose hydrolysis. International Journal of Biological Macromolecules. 2019, 139: 570-576.
44. Benwei Zhu, Kuikui Li, Wenxia Wang, Limin Ning, Haidong Tan, Xiaoming Zhao, Heng Yin. Preparation of trisaccharides from alginate by a novel alginate lyase Alg7A from marine bacterium Vibrio sp. W13. International Journal of Biological Macromolecules. 2019, 139: 879-885.
45. Xian-yu Zhu, Yong Zhao, Huai-dong Zhang, Wen-xia Wang, Hai-hua Cong, Heng Yin. Characterization of the Specific Mode of Action of a Chitin Deacetylase and Separation of the Partially Acetylated Chitosan Oligosaccharides. Marine Drugs. 2019, 17: 74.
46. Junyan Ma, Qian Li, Haidong Tan, Hao Jiang, Kuikui Li, Lihua Zhang, Quan Shi, Heng Yin. Unique N-glycosylation of a recombinant exo-inulinase from Kluyveromyces cicerisporus and its effect on enzymatic activity and thermostability. Journal Of Biological Engineering. 2019, 13: 81.
47. Fengqin Gong, Qishun Liu, Haidong Tan, Tang Li, Chengyu Tan, Heng Yin. Cloning, expression and characterization of a novel (2R,3R) -2,3-butanediol dehydrogenase from Bacillus thuringiensis. Biotechnology, Biocatalysis and Agricultural. 2019, 22(101): 372.
48. Ming Sun, Chu Sun, Hongguo Xie, Shenggang Yan, Heng Yin. A simple method to calculate the degree of polymerization of alginate oligosaccharides and low molecular weight alginates. Carbohydrate Research. 2019, 486(107): 856.
